Gentaur Cell Culture

European Collection of Authenticated Cell Cultures

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings.

The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business.

The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

Cell Culture Industries
Cell Culture Industries

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).



Cell Culture Plate

CCP01000-00-CellCulturePlate6Well2CellCulturePlate96Well2 Cell Culture Plate,6 Well*2Cell Culture Plate,96 Well*2
EUR 1.09

Water for Cell Culture

TBS5050 500ml
EUR 10

T225 Cell Culture Flask

P0230 25/case
EUR 185

Cell Culture Grade Water

25-511 6 x 500 mL/Unit
EUR 52.91
Description: Cell Culture Tested, Sterile

Cell Culture Grade Water

25-511B 6 x 1000 mL
EUR 85.86
Description: Cell Culture Tested, Sterile

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 148.8

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price


IB42010 1L
EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE


IB42011 6x1L
EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE


IB42012 12x1L
EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE


IB42020 2L
EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE


IB42021 6x2L
EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE


IB42030 10L
EUR 194.72


63-0600 each Ask for price

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 8 rxn
EUR 499

25 cm2 Cell Culture Flask

CCF00025-00-10Bag 10 / Bag
EUR 1.09

25 cm2 Cell Culture Flask

CCF00025-200Case 200 / Case
EUR 186.39

75 cm2 Cell Culture Flask

CCF00075-00-5Bag 5 / Bag
EUR 1.09

75 cm2 Cell Culture Flask

CCF00075-100Case 100 / Case
EUR 176.04


91-200-80 1/pk
EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

175 cm2 Cell Culture Flask

CCF00175-40Case 40 / Case
EUR 119.36

225 cm2 Cell Culture Flask

CCF00225-25Case 25 / Case
EUR 93.2

Cell Culture Plate, 6 Well

CCP01006-50Case 50 / Case
EUR 61.59

Cell Culture Plate, 96 Well

CCP01096-100Case 100 / Case
EUR 154.24

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 116.4


91-200-78 1/pk
EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts


91-200-79 1/pk
EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts


91-200-81 1/pk
EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1336.8

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 571.2

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 142.8

6-Well Cell Culture Plates

25-105 100 Plates/Unit
EUR 119.75
Description: Flat Bottom Wells, TC Treated

HEPES. cell culture tested - kg

PM-B2093/1000 kg
EUR 146.69

6-well Cell Culture Plates

abx090688-100l 100 µl
EUR 125

6-well Cell Culture Plates

abx090688-1ml 1 ml Ask for price

6-well Cell Culture Plates

abx090688-200l 200 µl
EUR 306.25

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