European Collection of Authenticated Cell Cultures

• Marie
• December 1, 2021
• 0

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings.

The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business.

The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).

 

 

ExoStep Cell Culture
ExoS-25-C9	ImmunoStep	25 test	EUR 660.6

Hepes Cell Culture Grade
AK3268-0250	Akron Biotech	250g	Ask for price

Hepes Cell Culture Grade
AK3268-0500	Akron Biotech	500g	Ask for price

Hepes Cell Culture Grade
AK3268-1000	Akron Biotech	1kg	Ask for price

Ethanolamine, Cell Culture tested
CA071-010	GenDepot	100ml	EUR 116.4

Water, Cell Culture Grade
CA018-600	GenDepot	6x1000ml	EUR 148.8

ROCKER CELL CULTURE BAG, 20L
91-200-78	CORNING	1/pk	EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L
91-200-79	CORNING	1/pk	EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 2L
91-200-80	CORNING	1/pk	EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L
91-200-81	CORNING	1/pk	EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

6-well Cell Culture Plates
abx090688-50plates	Abbexa	50 plates	EUR 571.2

6-well Cell Culture Plates
abx090688-6plates	Abbexa	6 plates	EUR 142.8

CardioDISC (Scaffold for cell culture)
C-01-01-EX	Sceti	12 sheets	Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

Mouse ES Cell Culture Media
M16101	Neuromics	500 mL	EUR 1336.8

UltraPure Water, Cell Culture Tested
W9200-045	GenDepot	450ml	EUR 96

Gibco CHO Cell Culture Medium
220253	Scientific Laboratory Supplies	EACH	EUR 50.05

500mL Water Cell Culture Grade
25-055-CV	Scientific Laboratory Supplies	PK6	EUR 52.67

Greiner culture flasks tissue culture treated
C6231-200EA	Scientific Laboratory Supplies	PK200	EUR 189.24

Gelatin Solution , 0.1%, Cell culture tested
CA087-100	GenDepot	1L	EUR 116.4

Ceraplex, Premium Cell Culture Suplement(10X)
C3318-010	GenDepot	100ml	Ask for price

Ceraplex, Premium Cell Culture Suplement(10X)
C3318-050	GenDepot	500ml	Ask for price

Ampicillin sodium salt, cell culture grade
GA0283-100G	Glentham Life Sciences	100 g	EUR 265.2

Ampicillin sodium salt, cell culture grade
GA0283-25G	Glentham Life Sciences	25 g	EUR 122.4

Ampicillin sodium salt, cell culture grade
GA0283-5G	Glentham Life Sciences	5 g	EUR 64.8

Tranfferrin, Human Recombinant, Cell culture tested
T4600-100	GenDepot	100mg	EUR 303.6

Tranfferrin, Human Recombinant, Cell culture tested
T4600-101	GenDepot	1g	EUR 1226.4

Tranfferrin, Human Recombinant, Cell culture tested
T4600-110	GenDepot	10g	EUR 9456

Multifuge X4R Pro cell culture package
75016066	Scientific Laboratory Supplies	EACH	EUR 19671.84

Multifuge X4 Pro cell culture package
75016067	Scientific Laboratory Supplies	EACH	EUR 14603.4

BD Biocoat 10cm cell culture dish
354469	Scientific Laboratory Supplies	PK10	EUR 101.46

1L Water Cell Culture Grade WFI
25-055-CM	Scientific Laboratory Supplies	PK6	EUR 80.94

3D Cell Culture Matrix BME Kit
K518-100	Biovision	each	EUR 744

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case
SP31171	Bio Basic	1CS, 500UNIT	EUR 230.89

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case
SP31212	Bio Basic	1CS, 200UNIT	EUR 162.61

3D Cell Culture Non-Enzymatic Cell Harvesting Kit
K982-100	Biovision	each	EUR 326.4

ExoStepTM Culture + Standard
ExoS-25-CST9	ImmunoStep	25 test	EUR 871.2

culture flask 250ml
FLA4286	Scientific Laboratory Supplies	PK10	EUR 251.21

culture flask 500ml
FLA4288	Scientific Laboratory Supplies	PK5	EUR 123.67