European Collection of Authenticated Cell Cultures

• Marie
• December 1, 2021
• 0

Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media change. Monolayer tissue culture has since been widely adopted as a routine method for assaying cell activity. Although glass was initially the predominant culture substrate of choice, clear polystyrene has taken precedence due to its cost efficiency, ease of visual analysis, and ability to promote cell adherence without the use of coatings.

The European Collection of Authenticated Cell Cultures (ECACC) was established in 1984 as a cell
culture collection to service the research community and provide an International Depository Authority
recognised patent depository for Europe. Over the last three decades ECACC has expanded and diversified to become one of the premier collections of authenticated cell cultures in the world and this remains the core of ECACC’s business.

The collections currently hold over 40,000 cell lines representing 45 different species, 50 tissue types, 300 HLA types, 450 monoclonal antibodies and at least 800 genetic disorders.
The development and maintenance of such a diverse collection has inevitably produced a high level of
specialist knowledge and this has enabled ECACC to position itself as a centre of expertise in all aspects
of cell culture. ECACC has developed a comprehensive range of cell culture services and diversified into
new product areas such as extracted from cell lines.

ECACC is the largest of four collections of clinically significant organisms, maintained by Public Health
England (PHE). The other collections are the National Collection of Type Cultures (NCTC), the National
Collection of Pathogenic Viruses (NCPV), and the National Collection of Pathogenic Fungi (NCPF).

Design and Equipment for the Cell Culture Laboratory 2.1 Laboratory Design

Perhaps one of the most undervalued aspects of tissue culture is the need
to design the facility to ensure that good quality material is produced in a
safe and efficient manner. Most tissue culture is undertaken in laboratories
that have been adapted for the purpose and in conditions that are not ideal.
However, as long as a few basic guidelines are adopted this should not
compromise the work.

Traditional methods of central nervous system culture

Cell and tissue culture techniques have been central to neuroscience research and the advancing knowledge of key mechanisms underpinning central nervous system (CNS) development, function and malfunction. These methods have permitted investigation of cellular level functions that would be infeasible to carry out routinely in vivo. Not only are animal studies often more time consuming and expensive, the complexity of the in vivo environment can make it difficult to isolate and control confounding variables in order to investigate specific cell and molecular features. In some cases inaccessibility of relevant tissue renders such studies completely impossible.

One of the earliest reports of in vitro CNS cell culture was carried out by Harrison, who observed neurite extension in fragments of frog medullary tube cultured using the hanging drop method, which involves the inversion of tissue-containing solution that has been placed on a cover slip to create a hanging drop [1]. The study was pivotal in representing one of the first examples of CNS tissue being grown ex vivo. By the mid-20th century, human CNS tissue culture had become more common and was often performed in either hanging drop or roller tube formats [2,3]. Studies carried out using roller tube methods involved growing neural tissue explants on rotating surfaces to allow alternating exposure to culture medium and gaseous phases.

In 1955, Cavanaugh became the first to report the application of monolayer culture using neurons derived from chick embryos and then, two decades later by the mid-1970s, monolayer CNS tissue culture had begun to gain popularity. The monolayer cell culture technique relied on dissociation of brain tissues using trypsin or collagenase to form single cell suspensions which were then grown on various glass or plastic substrates. These studies were used to identify morphological, molecular and electrophysiological features of neurons and other CNS relevant cell types in vitro (reviewed in).

 

 

Cell Culture Plate
CCP01000-00-CellCulturePlate6Well2CellCulturePlate96Well2	Vazyme	Cell Culture Plate，6 Well*2Cell Culture Plate，96 Well*2	EUR 1.09

10X HBSS Cell culture
IBS-BH011	iNtRON Biotechnology Inc	1L	EUR 35

Water for Cell Culture
IBS-CB016	iNtRON Biotechnology Inc	1L	EUR 35

Water for Cell Culture
TBS5050	Tribioscience	500ml	EUR 10

T175 Cell Culture Flask
P0220	ABM	40/case	EUR 215

T225 Cell Culture Flask
P0230	ABM	25/case	EUR 185

Cell Culture Grade Water
25-511	Genesee Scientific	6 x 500 mL/Unit	EUR 52.91
Description: Cell Culture Tested, Sterile

Cell Culture Grade Water
25-511B	Genesee Scientific	6 x 1000 mL	EUR 85.86
Description: Cell Culture Tested, Sterile

Hepes Cell Culture Grade
AK3268-0250	Akron Biotech	250g	Ask for price

Hepes Cell Culture Grade
AK3268-0500	Akron Biotech	500g	Ask for price

Hepes Cell Culture Grade
AK3268-1000	Akron Biotech	1kg	Ask for price

Water, Cell Culture Grade
CA018-600	GenDepot	6x1000ml	EUR 148.8

CELL CULTURE GRADE WATER
IB42010	IBI Scientific	1L	EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42011	IBI Scientific	6x1L	EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42012	IBI Scientific	12x1L	EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42020	IBI Scientific	2L	EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42021	IBI Scientific	6x2L	EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42030	IBI Scientific	10L	EUR 194.72
Description: JUG W/HANDL 0.1 MICRON STERILE

CRFK CELL TUBE CULTURE
63-0600	Quidel	each	Ask for price

100 mm Cell Culture Dish
P0300	ABM	300/case	EUR 250

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)
P770	101Bio	8 rxn	EUR 499

25 cm2 Cell Culture Flask
CCF00025-00-10Bag	Vazyme	10 / Bag	EUR 1.09

25 cm2 Cell Culture Flask
CCF00025-200Case	Vazyme	200 / Case	EUR 186.39

75 cm2 Cell Culture Flask
CCF00075-00-5Bag	Vazyme	5 / Bag	EUR 1.09

75 cm2 Cell Culture Flask
CCF00075-100Case	Vazyme	100 / Case	EUR 176.04

ROCKER CELL CULTURE BAG, 2L
91-200-80	CORNING	1/pk	EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

175 cm2 Cell Culture Flask
CCF00175-40Case	Vazyme	40 / Case	EUR 119.36

225 cm2 Cell Culture Flask
CCF00225-25Case	Vazyme	25 / Case	EUR 93.2

Cell Culture Plate, 6 Well
CCP01006-50Case	Vazyme	50 / Case	EUR 61.59

Cell Culture Plate, 96 Well
CCP01096-100Case	Vazyme	100 / Case	EUR 154.24

Ethanolamine, Cell Culture tested
CA071-010	GenDepot	100ml	EUR 116.4

ROCKER CELL CULTURE BAG, 20L
91-200-78	CORNING	1/pk	EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L
91-200-79	CORNING	1/pk	EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L
91-200-81	CORNING	1/pk	EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media
M16101	Neuromics	500 mL	EUR 1336.8

6-Well Cell Culture Plates
25-105	Genesee Scientific	100 Plates/Unit	EUR 119.75
Description: Flat Bottom Wells, TC Treated

6-well Cell Culture Plates
abx090688-100l	Abbexa	100 µl	EUR 125

6-well Cell Culture Plates
abx090688-1ml	Abbexa	1 ml	Ask for price