Lineage

genome SARS-CoV-2, B.1.1.7 sequences from randomly sampled residual

Summary

What is already known about this topic?

A more highly transmissible variant of SARS-CoV-2, B.1.1.7, has been detected in start highlight12end highlight U.S. states.

What is added by this report?

Modeling data indicate that B.1.1.7 has the potential to increase the U.S. pandemic trajectory in the coming months. CDC’s system for genomic surveillance and the effort to expand sequencing will increase the availability of timely U.S. genomic surveillance data.

 

We examined whole-genome SARS-CoV-2 sequences from randomly sampled residual materials obtained from community-based COVID-19 testing in England, collected between 1 October 2020 and 16 January 2021. These data included 31,390 B.1.1.7 sequences for which the time and location of sample collection were known. Over the same period, 52,795 non-VOC genomes were collected. VOC sequences were initially concentrated in London (n = 9,134), the South East (n = 5,609), and the East of England (n = 4,413), but is now widely distributed across England. Overall, we estimate the median posterior additive difference in growth rates between B.1.1.7 and co-circulating variants to be 0.69 per week (95% credible interval (CrI) 0.61–0.76) (Fig. 1a, Extended Data Fig. 1, Supplementary Methods section 2), and this difference was largest in November. However, in tandem with geographic expansion of the VOC and imposition of lockdown measures in 2021, this difference declined gradually to 0.43 per week (95% CrI 0.33–0.52) for the week ending 16 January.

 

What are the implications for public health practice?

The increased transmissibility of the B.1.1.7 variant warrants universal and increased compliance with mitigation strategies, including distancing and masking. Higher vaccination coverage might need to be achieved to protect the public. Genomic sequence analysis through the National SARS-CoV-2 Strain Surveillance program will enable a targeted approach to identifying variants of concern in the United States.

 

lineage b.1.1.7
lineage b.1.1.7

Abstract

The SARS-CoV-2 lineage B.1.1.7, designated variant of concern (VOC) 202012/01 by Public Health England1, was first identified in the UK in late summer to early autumn 20202. Whole-genome SARS-CoV-2 sequence data collected from community-based diagnostic testing for COVID-19 show an extremely rapid expansion of the B.1.1.7 lineage during autumn 2020, suggesting that it has a selective advantage. Here we show that changes in VOC frequency inferred from genetic data correspond closely to changes inferred by S gene target failures (SGTF) in community-based diagnostic PCR testing. Analysis of trends in SGTF and non-SGTF case numbers in local areas across England shows that B.1.1.7 has higher transmissibility than non-VOC lineages, even if it has a different latent period or generation time.

The SGTF data indicate a transient shift in the age composition of reported cases, with cases of B.1.1.7 including a larger share of under 20-year-olds than non-VOC cases. We estimated time-varying reproduction numbers for B.1.1.7 and co-circulating lineages using SGTF and genomic data. The best-supported models did not indicate a substantial difference in VOC transmissibility among different age groups, but all analyses agreed that B.1.1.7 has a substantial transmission advantage over other lineages, with a 50% to 100% higher reproduction number.

Main

The SARS-CoV-2 lineage B.1.1.7 spread rapidly across England between November 2020 and January 2021. This variant possesses a large number of non-synonymous substitutions of immunological importance2. The N501Y replacement on the spike protein has been shown to increase ACE2 binding3,4 and cell infectivity in animal models5, and the P618H replacement on the spike protein adjoins the furin-cleavage site6. B.1.1.7 also possesses a deletion at positions 69 and 70 of the spike protein (Δ69–70) that has been associated with failure of diagnostic tests using the ThermoFisher TaqPath probe, which targets the spike protein.

Although other variants with Δ69–70 are also circulating in the UK, the absence of detection of the S gene target in an otherwise positive PCR test appears to be a highly specific biomarker for the B.1.1.7 lineage. Data from national community testing in November 2020 showed a rapid increase in SGTF during PCR testing for SARS-CoV-2, coinciding with a rapid increase in the frequency of B.1.1.7 observed in genomic surveillance. The B.1.1.7 lineage was designated VOC 202012/01 by Public Health England (PHE) in December 2020.

 

Mixed Lineage Kinase 2 (MLK2) Antibody

20-abx121520
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

Mixed Lineage Kinase 2 (MLK2) Antibody

abx033764-400ul 400 ul
EUR 627.6

Mixed Lineage Kinase 2 (MLK2) Antibody

abx033764-80l 80 µl
EUR 343.2

Mixed Lineage Kinase 4 (MLK4) Antibody

abx033812-400ul 400 ul
EUR 627.6

Mixed Lineage Kinase 4 (MLK4) Antibody

abx033812-80l 80 µl
EUR 343.2

Mixed Lineage Kinase 2 (MLK2) Antibody

abx235214-100ug 100 ug
EUR 661.2

Mixed Lineage Kinase 3 (MLK3) Antibody

abx235215-100ug 100 ug
EUR 577.2

Mixed Lineage Kinase 4 (MLK4) Antibody

abx235216-100ug 100 ug
EUR 661.2

Mixed Lineage Kinase 2 (MLK2) Antibody

abx033764-100g 100 µg
EUR 250

Mixed Lineage Kinase 4 (MLK4) Antibody

abx033812-100g 100 µg
EUR 281.25

Mixed Lineage Kinase 2 (MLK2) Antibody

abx235214-100g 100 µg
EUR 350

Mixed Lineage Kinase 3 (MLK3) Antibody

abx235215-100g 100 µg
EUR 350

Mixed Lineage Kinase 4 (MLK4) Antibody

abx235216-100g 100 µg
EUR 350

Mixed Lineage Kinase 2 (MLK2) Antibody

abx375124-96tests 96 tests
EUR 337.5

Mixed lineage kinase domain-like protein

AP79175 1mg
EUR 2640

Mixed lineage kinase domain-like protein

AP85602 1mg
EUR 2640

PE anti-mouse CD93 (AA4.1, early B lineage)

E16FMP093-050U 50 μg
EUR 390
Description: Available in various conjugation types.

PE anti-mouse CD93 (AA4.1, early B lineage)

E16FMP093-200U 200 μg
EUR 1126.67
Description: Available in various conjugation types.

Human NPC Differentiation Medium, Neuron Lineage

cAP-60 100ml
EUR 73.26

APC anti-mouse CD93 (AA4.1, early B lineage)

E16FMA093-025U 25 μg
EUR 325
Description: Available in various conjugation types.

APC anti-mouse CD93 (AA4.1, early B lineage)

E16FMA093-100U 100 μg
EUR 1040
Description: Available in various conjugation types.

FITC anti-mouse CD93 (AA4.1, early B lineage)

E16FMF093-050U 50 μg
EUR 346.67
Description: Available in various conjugation types.

FITC anti-mouse CD93 (AA4.1, early B lineage)

E16FMF093-200U 200 μg
EUR 845
Description: Available in various conjugation types.

Human NPC Differentiation Medium, Astrocyte Lineage

cAP-59 100ml
EUR 61.38

Human mixed lineage kinase domain- like ELISA Kit

ELA-E1747h 96 Tests
EUR 988.8

OOTA00772-PANEL-25UG - Mouse Biotin Lineage Panel

OOTA00772-PANEL-25UG Panel-25ug
EUR 139

PE-Cy7 anti-mouse CD93 (AA4.1, early B lineage)

E16FMN093-025U 25 μg
EUR 411.67
Description: Available in various conjugation types.

PE-Cy7 anti-mouse CD93 (AA4.1, early B lineage)

E16FMN093-100U 100 μg
EUR 1235
Description: Available in various conjugation types.

OOTA00772-PANEL-100UG - Mouse Biotin Lineage Panel

OOTA00772-PANEL-100UG Panel-100ug
EUR 329

Oligodendrocyte Lineage Transcription Factor 2 Antibody

20-abx114262
  • Ask for price
  • Ask for price
  • 150 ul
  • 50 ul

Human NPC Differentiation Medium, Oligodendrocyte Lineage

cAP-61 100ml
EUR 61.38

Leave a Reply

Your email address will not be published. Required fields are marked *