Category: elisa reagents
Cell Stimulation Cocktail Protein Transport Inhibitor Cocktail
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The EZCell Protein Transport Inhibitor Cocktail (500X) is a cocktail composed of of Brefeldin A and Monensin, formulated in DMSO. Useful in assays that require prevention of protein transport to the extracellular space is required. Addition of this cocktail to cells in culture inhibits the intracellular protein secretory/transport pathway resulting in the accumulation of secreted …
Read MoreEuropean Collection of Authenticated Cell Cultures
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Conventional monolayer culture methods have also been referred to as two-dimensional (2D) because they require cell adherence to a flat surface, to which culture medium is applied allowing access to nutrients that enable cell survival and proliferation. While healthy cells remain attached to the surface, necrotic cells become detached and are easily removed during media …
Read MoreTMB Chromogen Solution (for ELISA)
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The assay can be performed directly with serum and in this case will measure total fructosamine in serum proteins. Alternatively, it is possible to separate albumin from other serum proteins by Affi-Gel blue chromatography and then to perform the colorimetric fructosamine assay. The assay is not sensitive to the labile Schiff’s bases (aldimines) or the …
Read MoreHRP antibody labeling protocol
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Direct detection is often a preferred method within these applications to avoid cross-species reactivity and to eliminate additional laborious wash and separation steps, especially in time-consuming protocols. However, directly conjugating HRP to the antibody or protein of choice can be difficult and labor-intensive if using traditional methodology. Our Lightning-Link HRP antibody labeling kit allows the direct conjugation of your antibody, protein, …
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