Cell Stimulation Cocktail Protein Transport Inhibitor Cocktail
The EZCell Protein Transport Inhibitor Cocktail (500X) is a cocktail composed of of Brefeldin A and Monensin, formulated in DMSO. Useful in assays that require prevention of protein transport to the extracellular space is required. Addition of this cocktail to cells in culture inhibits the intracellular protein secretory/transport pathway resulting in the accumulation of secreted proteins in the lumen of the endoplasmic reticulum and in the Golgi apparatus. This accumulation can then be detected by intracellular staining and flow cytometric analysis or Western blotting.
Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo.
The EZCell CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
Supplier: BioVision
The Cell Stimulation Cocktail (500X) is a ready-to-use cocktail useful to induce activation of many cell types to produce cytokines. This cocktail can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays. Each vial contains Protein Kinase C activator Phorbol 12-Myristate 13-Acetate (PMA) (40.5 µM) and the calcium ionophore, Ionomycin (670 µM) formulated in DMSO (500X).
Product Details
| Appearance | Liquid |
|---|---|
| Formulation | In DMSO |
| CAS # | See under individual products |
| Molecular Formula | See under individual products |
| Molecular Weight | See under individual products |
| Handling | Protect from air and light |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
| SKU | K932-500 |
|---|---|
| Size | 500 ul |
| Application | A Protein Transport Inhibitor cocktail |
| Cas no | See under individual products |
| Storage | -20C |
| Shipping Temp | Gel Pack |
Alternate Name : EZCell™ Cell Tracer Kit,CFDA Cell Tracer Kit,CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Kit,Cell Tracer Kit
Tag Line : Flow cytometry based assay to determine cell proliferation using CFDA SE dye
Summary : BioVisions EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.
EZCell? Calcium Detection Kit (Cell-Based) |
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| K432-50 | Biovision | each | EUR 679.2 |
EZCell? Intracellular Zinc Staining Kit (Cell-Based) |
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| K525-50 | Biovision | each | EUR 679.2 |
cleavage stimulation factor Antibody |
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| 35503-100ul | SAB | 100ul | EUR 468 |
Cleavage Stimulation Factor 1 Protein |
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| 20-abx260926 | Abbexa |
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anti-Cleavage stimulation factor 2 |
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| YF-PA11164 | Abfrontier | 50 ul | EUR 435.6 |
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Description: Mouse polyclonal to Cleavage stimulation factor 2 |
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anti-Cleavage stimulation factor 2 |
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| YF-PA11165 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to Cleavage stimulation factor 2 |
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anti-Cleavage stimulation factor 2 |
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| YF-PA11166 | Abfrontier | 100 ul | EUR 483.6 |
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Description: Rabbit polyclonal to Cleavage stimulation factor 2 |
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anti-Cleavage stimulation factor 2 |
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| YF-PA11167 | Abfrontier | 100 ug | EUR 483.6 |
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Description: Rabbit polyclonal to Cleavage stimulation factor 2 |
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anti-Cleavage stimulation factor 2 |
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| YF-PA23534 | Abfrontier | 50 ul | EUR 400.8 |
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Description: Mouse polyclonal to Cleavage stimulation factor 2 |
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anti-Cleavage stimulation factor 3 |
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| YF-PA23535 | Abfrontier | 50 ul | EUR 400.8 |
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Description: Mouse polyclonal to Cleavage stimulation factor 3 |
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Anti-cleavage stimulation factor (1C6) |
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| YF-MA12593 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to cleavage stimulation factor |
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EZCell? Phagocytosis Assay Kit (Green Zymosan) |
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| K397-100 | Biovision | each | EUR 516 |
EZCell? Phagocytosis Assay Kit (Red Zymosan) |
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| K398-100 | Biovision | each | EUR 516 |
EZCell? Glutathione Detection Kit (Blue Fluorescence) |
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| K504-100 | Biovision | each | EUR 457.2 |
EZCell? Direct Glucose Uptake Assay Kit |
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| K924-50 | Biovision | each | EUR 888 |
EZCell? Protein Transport Inhibitor Cocktail (500X) |
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| K932-500 | Biovision | each | EUR 235.2 |
EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm) |
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| K906-100 | Biovision | each | EUR 679.2 |
EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) |
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| K907-100 | Biovision | each | EUR 666 |
EZCell? Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) |
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| K909-12 | Biovision | each | EUR 548.4 |
EZCell? Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm) |
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| K910-12 | Biovision | each | EUR 535.2 |
EZCell? Cell Invasion Assay Kit (Laminin), 12-well, 8 µm |
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| K915-12 | Biovision | each | EUR 614.4 |
EZCell? Cell Invasion Assay (Collagen I), 96-well, 8 µm |
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| K916-100 | Biovision | each | EUR 692.4 |
EZCell? Cell Invasion Assay (Collagen IV), 96-well, 8 µm |
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| K918-100 | Biovision | each | EUR 679.2 |
Cleavage Stimulation Factor 1 (CSTF1) Antibody |
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| 20-abx137481 | Abbexa |
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Anti-Cleavage stimulation factor 2 (1D4) |
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| YF-MA10205 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to Cleavage stimulation factor 2 |
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EZCell? Phagocytosis Assay Kit (Green E. coli) |
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| K963-100 | Biovision | each | EUR 516 |
EZCell? Phagocytosis Assay Kit (Red E. coli) |
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| K964-100 | Biovision | each | EUR 502.8 |
Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody |
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| abx145028-100ug | Abbexa | 100 ug | EUR 469.2 |
Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody |
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| 20-abx124031 | Abbexa |
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Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody |
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| 20-abx125725 | Abbexa |
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Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody |
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| 20-abx004532 | Abbexa |
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Cleavage Stimulation Factor Subunit 2 (CSTF2) Antibody |
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| 20-abx006066 | Abbexa |
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Cleavage Stimulation Factor Subunit 2 (CSTF2) Antibody |
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| 20-abx013470 | Abbexa |
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Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody |
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| 20-abx013580 | Abbexa |
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Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody |
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| abx027771-400ul | Abbexa | 400 ul | EUR 627.6 |
Cleavage Stimulation Factor Subunit 3 (CSTF3) Antibody |
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| abx027771-80l | Abbexa | 80 µl | EUR 343.2 |
Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody |
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| abx232037-100ug | Abbexa | 100 ug | EUR 577.2 |
Cleavage Stimulation Factor Subunit 1 (CSTF1) Antibody |
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| abx232038-100ug | Abbexa | 100 ug | EUR 577.2 |
