biovision ezcell cocktail

Cell Stimulation Cocktail Protein Transport Inhibitor Cocktail

The EZCell Protein Transport Inhibitor Cocktail (500X) is a cocktail composed of of Brefeldin A and Monensin, formulated in DMSO. Useful in assays that require prevention of protein transport to the extracellular space is required. Addition of this cocktail to cells in culture inhibits the intracellular protein secretory/transport pathway resulting in the accumulation of secreted proteins in the lumen of the endoplasmic reticulum and in the Golgi apparatus. This accumulation can then be detected by intracellular staining and flow cytometric analysis or Western blotting.

Carboxyfluorescein Diacetate Succinimidyl Ester (CFDA SE) is a colorless, non-fluorescent, stable cell-permeable dye. Once it enters into the cells, the intracellular esterases remove the acetate groups to generate Carboxyfluorescein Succinimidyl Ester, which then covalently binds to lysine residues or other amines and produces a bright fluorescent conjugate. The fluorescent conjugate is stable and is retained inside the cells during cell division and hence it is an ideal tool for cell tracing studies, both in vitro and in vivo.

The EZCell CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.

 

Supplier: BioVision

Catalogue number: K930-100
Size: 100 µl

 

The Cell Stimulation Cocktail (500X) is a ready-to-use cocktail useful to induce activation of many cell types to produce cytokines. This cocktail can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays. Each vial contains Protein Kinase C activator Phorbol 12-Myristate 13-Acetate (PMA) (40.5 µM) and the calcium ionophore, Ionomycin (670 µM) formulated in DMSO (500X).

 

biovision ezcell cocktail
biovision ezcell cocktail

Product Details

Appearance Liquid
Formulation In DMSO
CAS # See under individual products
Molecular Formula See under individual products
Molecular Weight See under individual products
Handling Protect from air and light
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.
Description
The Cell Stimulation and Protein Transport Inhibitor Cocktail is a ready-to-use cocktail consisting of an optimized mixture of Phorbol 12-Myristate 13-Acetate (PMA), Ionomycin, Brefeldin A, and monesin. Formulated in DMSO (500X). PMA and Ionomycin in duce cytokine production in many cell types and Brefeldin A and Monensin act as protein transport inhibitors. This cocktail is used to induce cytokine production and inhibit their intracellular transport for flow cytometry detection.
More Information
SKU K932-500
Size 500 ul
Application A Protein Transport Inhibitor cocktail
Cas no See under individual products
Storage -20C
Shipping Temp Gel Pack

 

Alternate Name : EZCell™ Cell Tracer Kit,CFDA Cell Tracer Kit,CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Tracer Kit,EZCell™ CFDA SE Cell Kit,Cell Tracer Kit

Tag Line : Flow cytometry based assay to determine cell proliferation using CFDA SE dye

Summary : BioVision’s EZCell™ CFDA SE Cell Tracer Kit provides an easy, rapid and sensitive way to determine cell proliferation using CFDA SE dye. The dye can be inherited by the daughter cells for up to 8 generations. Following each cell division, the fluorescence intensity is halved, which can be quantified by measuring the fluorescence intensity at Ex/Em = 492/517 nm. The assay is non-radioactive, accurate and can be used for cell tracing studies.

Cell Stimulation MIX Kit

E-CK-A019-200Assays 200 Assays
EUR 160

Cell Stimulation MIX Kit

E-CK-A019-500Assays 500 Assays
EUR 250

Cell Stimulation MIX Kit

E-CK-A019-50Assays 50 Assays
EUR 60

Cell Stimulation MIX Kit

E-CK-A019-each each Ask for price

Cell Stimulation MIX Kit

MBS2570567-200Assays 200Assays
EUR 185

Cell Stimulation MIX Kit

MBS2570567-500Assays 500Assays
EUR 240

Cell Stimulation MIX Kit

MBS2570567-50Assays 50Assays
EUR 125

Cell Stimulation MIX Kit

MBS2570567-5x500Assays 5x500Assays
EUR 1065

Cell Stimulation Cocktail (500X) 100 uL

E16FXP048-100 100 μl
EUR 193
Description: Available in various conjugation types.

OOTA00786-100UL - Cell Stimulation Cocktail

OOTA00786-100UL 100uL
EUR 79

OOTA00786-400UL - Cell Stimulation Cocktail

OOTA00786-400UL 400uL
EUR 229

Cell Stimulation Cocktail (500X) 4 x 100 uL

E16FXP048-4x100 4x100 μl
EUR 559.33
Description: Available in various conjugation types.

Cell stimulation and Protein Transport Inhibitor Kit

E-CK-A091-100Assays 100 Assays
EUR 125

Cell stimulation and Protein Transport Inhibitor Kit

E-CK-A091-200Assays 200 Assays
EUR 200

Cell stimulation and Protein Transport Inhibitor Kit

E-CK-A091-500Assays 500 Assays
EUR 350

Cell stimulation and Protein Transport Inhibitor Kit

E-CK-A091-50Assays 50 Assays
EUR 80

Cell stimulation and Protein Transport Inhibitor Kit

MBS2570569-100Assays 100Assays
EUR 225

Cell stimulation and Protein Transport Inhibitor Kit

MBS2570569-200Assays 200Assays
EUR 275

Cell stimulation and Protein Transport Inhibitor Kit

MBS2570569-500Assays 500Assays
EUR 365

Cell stimulation and Protein Transport Inhibitor Kit

MBS2570569-50Assays 50Assays
EUR 195

Cell stimulation and Protein Transport Inhibitor Kit

MBS2570569-5x500Assays 5x500Assays
EUR 1660

Cell Stimulation and Protein Transport Inhibitor Cocktail

MBS697185-01mL 0.1mL
EUR 195

Cell Stimulation and Protein Transport Inhibitor Cocktail

MBS697185-5x01mL 5x0.1mL
EUR 825

EZCell? Calcium Detection Kit (Cell-Based)

K432-50 each
EUR 679.2

EZCell? Cell Invasion Assay Kit (Laminin), 12-well, 8 µm

K915-12 each
EUR 614.4

EZCell? Cell Invasion Assay (Collagen I), 96-well, 8 µm

K916-100 each
EUR 692.4

EZCell? Cell Invasion Assay (Collagen IV), 96-well, 8 µm

K918-100 each
EUR 679.2

EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 8 µm)

K906-100 each
EUR 679.2

EZCell? Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm)

K907-100 each
EUR 666

EZCell? Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm)

K909-12 each
EUR 548.4

EZCell? Cell Migration/Chemotaxis Assay Kit (24-well, 5 µm)

K910-12 each
EUR 535.2

Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) 100 uL

E16FXP049-100 100 μl
EUR 323.17
Description: Available in various conjugation types.

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50 each
EUR 679.2

Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) 4 x 100 uL

E16FXP049-4x100 4x100 μl
EUR 849
Description: Available in various conjugation types.

cleavage stimulation factor Antibody

35503 100ul
EUR 479

cleavage stimulation factor Antibody

35503-100ul 100ul
EUR 468

cleavage stimulation factor Antibody

MBS9410890-01mL 0.1mL
EUR 420

cleavage stimulation factor Antibody

MBS9410890-5x01mL 5x0.1mL
EUR 1740

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