Tag: cell culture 101
TMB Chromogen Solution (for ELISA)
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The assay can be performed directly with serum and in this case will measure total fructosamine in serum proteins. Alternatively, it is possible to separate albumin from other serum proteins by Affi-Gel blue chromatography and then to perform the colorimetric fructosamine assay. The assay is not sensitive to the labile Schiff’s bases (aldimines) or the …
Read MoreHRP antibody labeling protocol
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Direct detection is often a preferred method within these applications to avoid cross-species reactivity and to eliminate additional laborious wash and separation steps, especially in time-consuming protocols. However, directly conjugating HRP to the antibody or protein of choice can be difficult and labor-intensive if using traditional methodology. Our Lightning-Link HRP antibody labeling kit allows the direct conjugation of your antibody, protein, …
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