
HRP antibody labeling protocol
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Direct detection is often a preferred method within these applications to avoid cross-species reactivity and to eliminate additional laborious wash and separation steps, especially in time-consuming protocols. However, directly conjugating HRP to the antibody or protein of choice can be difficult and labor-intensive if using traditional methodology.
Our Lightning-Link HRP antibody labeling kit allows the direct conjugation of your antibody, protein, or peptide to HRP for use in any application. The kit only requires 30 seconds of hands-on time, and conjugates are ready to use after only 3 hours. Furthermore, there is no need for extra wash or separation steps post conjugation.
Directly label your primary antibody with HRP
Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which can be conjugated to antibodies and proteins for use in a variety of applications.
The enzyme label can be visualized through chromogenic reactions. For example, diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted into a water-insoluble brown pigment. Other substrates that can be used to measure horseradish peroxidase activity include ABTS, TMB, and TMBUS.
HRP is a popular detection label used in research. Antibody-HRP conjugates are commonly used in ELISA, IHC, and western blotting. HRP can be conjugated to the primary antibody for direct detection or secondary antibody for indirect detection.

Benefits of using Lightning-Link HRP
- Quick and easy – 30 seconds of hands-on time
- Adjustable ratios of antibody to HRP, between 1:1 and 1:4
- No separation steps involved, recover 100% of your antibody or protein
- Potentially more sensitive when compared to traditional methods – so less antibody required (see figure 1 below)
- Label from as little as 10 µg – scale up to a gram or more.
Abcam’s HRP secondaries advantages are:
- High sensitivity – detect low-abundant targets with high signal-to-noise ratios due to an optimal number of HRP molecules per antibody.
- Robustness – can be used at dilutions ranging from 1/2,000 to 1/20,000 and provide high specificity and minimal background.
- Stability – they are supplied in a liquid format that is ready-to-use and can be stored at 4°C.
- Flexibility – compatible with chromogenic, fluorogenic and chemiluminescent substrates.
- Cost-effective – use less conjugate per assay and perform more blots.

Anti-mouse HRP secondary antibodies
Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.
Host | Non-preadsorbed* | Preadsorbed* |
Goat | Anti-mouse HRP (IgG H&L) | Yes |
Anti-mouse IgG Fc HRP | Yes | |
Anti-mouse IgG1 heavy chain HRP | Yes | |
Anti-mouse IgG2a heavy chain HRP | Yes | |
Anti-mouse IgG2b heavy chain HRP | Yes | |
Anti-mouse IgG2c heavy chain HRP | Yes | |
Anti-mouse IgG3 heavy chain HRP | Yes | |
Anti-mouse IgM mu chain HRP | Yes | |
Anti-mouse IgA alpha chain HRP | Yes | |
Anti-mouse IgG+IgM HRP (H&L) | Yes | |
Anti-mouse IgG+IgM+IgA HRP (H&L) | Yes | |
F(ab) Anti-mouse HRP (IgG H&L) | No | |
F(ab’)2 Anti-mouse HRP (IgG H&L) | Yes | |
F(ab’)2 Anti-mouse IgG Fc HRP | Yes | |
F(ab’)2 Anti-mouse IgG F(ab’)2 HRP | Yes | |
F(ab’)2 Anti-mouse IgM mu chain HRP | Yes | |
F(ab’)2 Anti-mouse IgG+IgM+IgA HRP (H&L) | Yes | |
Rabbit | Anti-mouse HRP (IgG H&L) | Yes |
F(ab’)2 Anti-mouse HRP (IgG H&L) | Yes | |
Donkey | Anti-mouse HRP (IgG H&L) | Yes |
F(ab’)2 Anti-mouse HRP (IgG H&L) | Yes |
* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross-reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow-through.
HRP antibody |
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10R-P156a | Fitzgerald | 1 mg | EUR 470.4 |
Description: Mouse monoclonal HRP antibody |
HRP antibody |
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20R-3010 | Fitzgerald | 100 µg | EUR 776.4 |
Description: Rabbit polyclonal HRP antibody |
HRP antibody |
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20C-CR7015GP | Fitzgerald | 500 ul | EUR 96 |
Description: Goat polyclonal HRP antibody |
HRP Antibody |
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abx015753-100ul | Abbexa | 100 ul | EUR 493.2 |
GST HRP Antibody |
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48050-100ul | SAB | 100ul | EUR 399.6 |
GST HRP Antibody |
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48050-50ul | SAB | 50ul | EUR 286.8 |
I79_001071 Antibody (HRP) |
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20-abx333649 | Abbexa |
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HA antibody (HRP) |
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60R-1086 | Fitzgerald | 1 ml | EUR 609.6 |
Description: Affinity purified Chicken polyclonal HA antibody (HRP) |
P Antibody (HRP) |
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20-abx300952 | Abbexa |
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anti- HRP 2 antibody |
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FNab04012 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against HRP 2 |
HA Tag HRP Antibody |
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48052-100ul | SAB | 100ul | EUR 399.6 |
HA Tag HRP Antibody |
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48052-50ul | SAB | 50ul | EUR 286.8 |
DTL Antibody (HRP) |
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20-abx315547 | Abbexa |
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GST Antibody (HRP) |
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abx448871-100ug | Abbexa | 100 ug | EUR 710.4 |
CBP Antibody (HRP) |
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abx449069-100ug | Abbexa | 100 ug | EUR 710.4 |
CBP Antibody (HRP) |
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abx449087-100ug | Abbexa | 100 ug | EUR 710.4 |
BAD Antibody (HRP) |
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abx449465-100ug | Abbexa | 100 ug | EUR 710.4 |
BAD Antibody (HRP) |
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abx449483-100ug | Abbexa | 100 ug | EUR 710.4 |
p53 Antibody (HRP) |
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abx446377-100ug | Abbexa | 100 ug | EUR 678 |
RAS Antibody (HRP) |
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abx447023-100ug | Abbexa | 100 ug | EUR 610.8 |
BSA antibody (HRP) |
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70R-AS002 | Fitzgerald | 1 ml | EUR 327.6 |
Description: Affinity purified Sheep polyclonal BSA antibody (HRP) |
IL4 antibody (HRP) |
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60R-1894 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal IL4 antibody (HRP) |
MIF antibody (HRP) |
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60R-1970 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal MIF antibody (HRP) |
C1D antibody (HRP) |
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60R-1981 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal C1D antibody (HRP) |
IL8 antibody (HRP) |
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60R-2006 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal IL8 antibody (HRP) |
APP antibody (HRP) |
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60R-2041 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal APP antibody (HRP) |
IL6 antibody (HRP) |
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60R-2100 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal IL6 antibody (HRP) |
FTL antibody (HRP) |
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60R-2139 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal FTL antibody (HRP) |
FUS antibody (HRP) |
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60R-2170 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal FUS antibody (HRP) |
DJ1 antibody (HRP) |
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60R-2218 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal DJ1 antibody (HRP) |
NSF antibody (HRP) |
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60R-2269 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal NSF antibody (HRP) |
p53 antibody (HRP) |
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60R-2272 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal p53 antibody (HRP) |
CRP Antibody, HRP |
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60R-2349 | Fitzgerald | 100 ug | EUR 398.4 |
Description: Rabbit polyclonal CRP antibody (HRP) |
GFP antibody (HRP) |
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60R-GG002hrp | Fitzgerald | 1 mg | EUR 988.8 |
Description: Goat polyclonal GFP antibody (HRP) conjugated |
VWF antibody (HRP) |
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60R-VG002hrp | Fitzgerald | 150 ug | EUR 429.6 |
Description: Goat polyclonal VWF antibody (HRP) conjugated |