HRP antibody labeling protocol

HRP antibody labeling protocol

Direct detection is often a preferred method within these applications to avoid cross-species reactivity and to eliminate additional laborious wash and separation steps, especially in time-consuming protocols. However, directly conjugating HRP to the antibody or protein of choice can be difficult and labor-intensive if using traditional methodology.

Our Lightning-Link HRP antibody labeling kit allows the direct conjugation of your antibody, protein, or peptide to HRP for use in any application. The kit only requires 30 seconds of hands-on time, and conjugates are ready to use after only 3 hours. Furthermore, there is no need for extra wash or separation steps post conjugation.

​Directly label your primary antibody with HRP

​​​Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which can be conjugated to antibodies and proteins for use in a variety of applications.

The enzyme label can be visualized through chromogenic reactions. For example, diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted into a water-insoluble brown pigment. Other substrates that can be used to measure horseradish peroxidase activity include ABTS, TMB, and TMBUS.

HRP is a popular detection label used in research. Antibody-HRP conjugates are commonly used in ELISA, IHC, and western blotting. HRP can be conjugated to the primary antibody for direct detection or secondary antibody for indirect detection.

 

hrp conjugated antibody
hrp conjugated antibody

Benefits of using Lightning-Link HRP

  • Quick and easy – 30 seconds of hands-on time
  • Adjustable ratios of antibody to HRP, between 1:1 and 1:4
  • No separation steps involved, recover 100% of your antibody or protein
  • Potentially more sensitive when compared to traditional methods – so less antibody required (see figure 1 below)
  • Label from as little as 10 µg – scale up to a gram or more.

Abcam’s HRP secondaries advantages are:

  • High sensitivity – detect low-abundant targets with high signal-to-noise ratios due to an optimal number of HRP molecules per antibody.
  • Robustness – can be used at dilutions ranging from 1/2,000 to 1/20,000 and provide high specificity and minimal background.
  • Stability – they are supplied in a liquid format that is ready-to-use and can be stored at 4°C.
  • Flexibility – compatible with chromogenic, fluorogenic and chemiluminescent substrates.
  • Cost-effective – use less conjugate per assay and perform more blots.
hrp conjugated antibody
hrp conjugated antibody

Anti-mouse HRP secondary antibodies

Our secondary antibody selection guide will help you choose the most appropriate secondary antibody for your experiment. Read it now to learn more about immunoglobulins classes and subclasses, preadsorbed secondary antibodies and F(ab) or F(ab’)2 fragment secondary antibodies.

Host Non-preadsorbed* Preadsorbed*
Goat Anti-mouse HRP (IgG H&L) Yes
Anti-mouse IgG Fc HRP Yes
Anti-mouse IgG1 heavy chain HRP Yes
Anti-mouse IgG2a heavy chain HRP Yes
Anti-mouse IgG2b heavy chain HRP Yes
Anti-mouse IgG2c heavy chain HRP Yes
Anti-mouse IgG3 heavy chain HRP Yes
Anti-mouse IgM mu chain HRP Yes
Anti-mouse IgA alpha chain HRP Yes
Anti-mouse IgG+IgM HRP (H&L) Yes
Anti-mouse IgG+IgM+IgA HRP (H&L) Yes
F(ab) Anti-mouse HRP (IgG H&L) No
F(ab’)2 Anti-mouse HRP (IgG H&L) Yes
F(ab’)2 Anti-mouse IgG Fc HRP Yes
F(ab’)2 Anti-mouse IgG F(ab’)2 HRP Yes
F(ab’)2 Anti-mouse IgM mu chain HRP Yes
F(ab’)2 Anti-mouse IgG+IgM+IgA HRP (H&L) Yes
Rabbit Anti-mouse HRP (IgG H&L) Yes
F(ab’)2 Anti-mouse HRP (IgG H&L) Yes
Donkey Anti-mouse HRP (IgG H&L) Yes
F(ab’)2 Anti-mouse HRP (IgG H&L) Yes

* Preadsorbed (cross-adsorbed) antibodies go through an extra purification step to minimize species cross-reactivity and background. The process involves passaging of the antibody solution through a column containing immobilized serum proteins from potentially cross-reactive species. Nonspecific cross-reactive secondary antibodies remain bound to the proteins in the column, while highly specific secondary antibodies are recovered from the flow-through.

 

Cholesterol (CH) Polyclonal Antibody (General), HRP

4-PAB701Ge01-HRP
  • EUR 362.40
  • EUR 3182.40
  • EUR 907.20
  • EUR 452.40
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Cholesterol (CH). This antibody is labeled with HRP.

Glycocalicin (GC) Polyclonal Antibody (Human), HRP

4-PAB710Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Glycocalicin (GC). This antibody is labeled with HRP.

Fascin (FSCN) Polyclonal Antibody (Human), HRP

4-PAB757Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Fascin (FSCN). This antibody is labeled with HRP.

Fascin (FSCN) Polyclonal Antibody (Mouse), HRP

4-PAB757Mu01-HRP
  • EUR 373.20
  • EUR 3350.40
  • EUR 949.20
  • EUR 469.20
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Fascin (FSCN). This antibody is labeled with HRP.

Neprilysin (NEP) Polyclonal Antibody (Mouse), HRP

4-PAB785Mu01-HRP
  • EUR 373.20
  • EUR 3350.40
  • EUR 949.20
  • EUR 469.20
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Neprilysin (NEP). This antibody is labeled with HRP.

Neprilysin (NEP) Polyclonal Antibody (Rat), HRP

4-PAB785Ra01-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with HRP.

Neprilysin (NEP) Polyclonal Antibody (Rat), HRP

4-PAB785Ra02-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with HRP.

Arginine (Arg) Polyclonal Antibody (General), HRP

4-PAB938Ge01-HRP
  • EUR 360.00
  • EUR 3163.20
  • EUR 902.40
  • EUR 451.20
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Arginine (Arg). This antibody is labeled with HRP.

Epiregulin (EREG) Polyclonal Antibody (Rat), HRP

4-PAB945Ra01-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Epiregulin (EREG). This antibody is labeled with HRP.

Acrosin (ACR) Polyclonal Antibody (Rat), HRP

4-PAB947Ra01-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Acrosin (ACR). This antibody is labeled with HRP.

Hepcidin (Hepc) Polyclonal Antibody (Bovine), HRP

4-PAB979Bo01-HRP
  • EUR 411.60
  • EUR 3894.00
  • EUR 1086.00
  • EUR 524.40
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Hepcidin (Hepc). This antibody is labeled with HRP.

Hepcidin (Hepc) Polyclonal Antibody (Human), HRP

4-PAB979Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hepcidin (Hepc). This antibody is labeled with HRP.

Hepcidin (Hepc) Polyclonal Antibody (Mouse), HRP

4-PAB979Mu01-HRP
  • EUR 373.20
  • EUR 3350.40
  • EUR 949.20
  • EUR 469.20
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Hepcidin (Hepc). This antibody is labeled with HRP.

Hepcidin (Hepc) Polyclonal Antibody (Pig), HRP

4-PAB979Po01-HRP
  • EUR 411.60
  • EUR 3894.00
  • EUR 1086.00
  • EUR 524.40
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Pig Hepcidin (Hepc). This antibody is labeled with HRP.

Hepcidin (Hepc) Polyclonal Antibody (Rat), HRP

4-PAB979Ra01-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Hepcidin (Hepc). This antibody is labeled with HRP.

Hemopexin (HPX) Polyclonal Antibody (Human), HRP

4-PAB986Hu01-HRP
  • EUR 332.40
  • EUR 2770.80
  • EUR 805.20
  • EUR 411.60
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hemopexin (HPX). This antibody is labeled with HRP.

Hemopexin (HPX) Polyclonal Antibody (Human), HRP

4-PAB986Hu02-HRP
  • EUR 332.40
  • EUR 2770.80
  • EUR 805.20
  • EUR 411.60
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hemopexin (HPX). This antibody is labeled with HRP.

Hemopexin (HPX) Polyclonal Antibody (Rat), HRP

4-PAB986Ra01-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Hemopexin (HPX). This antibody is labeled with HRP.

Hemopexin (HPX) Polyclonal Antibody (Rat), HRP

4-PAB986Ra02-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Hemopexin (HPX). This antibody is labeled with HRP.

Hemojuvelin (HJV) Polyclonal Antibody (Human), HRP

4-PAB995Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Hemojuvelin (HJV). This antibody is labeled with HRP.

Lipopolysaccharide (LPS) Polyclonal Antibody (General), HRP

4-PAB526Ge01-HRP
  • EUR 362.40
  • EUR 3182.40
  • EUR 907.20
  • EUR 452.40
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Lipopolysaccharide (LPS). This antibody is labeled with HRP.

Palladin (PALLD) Polyclonal Antibody (Human), HRP

4-PAB590Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Palladin (PALLD). This antibody is labeled with HRP.

Neuraminidase (NEU) Polyclonal Antibody (Human), HRP

4-PAB611Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Neuraminidase (NEU). This antibody is labeled with HRP.

Myostatin (MSTN) Polyclonal Antibody (Bovine), HRP

4-PAB653Bo01-HRP
  • EUR 411.60
  • EUR 3894.00
  • EUR 1086.00
  • EUR 524.40
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Myostatin (MSTN). This antibody is labeled with HRP.

Myostatin (MSTN) Polyclonal Antibody (Human), HRP

4-PAB653Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Myostatin (MSTN). This antibody is labeled with HRP.

Myostatin (MSTN) Polyclonal Antibody (Mouse), HRP

4-PAB653Mu01-HRP
  • EUR 373.20
  • EUR 3350.40
  • EUR 949.20
  • EUR 469.20
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Myostatin (MSTN). This antibody is labeled with HRP.

Myostatin (MSTN) Polyclonal Antibody (Pig), HRP

4-PAB653Po01-HRP
  • EUR 411.60
  • EUR 3894.00
  • EUR 1086.00
  • EUR 524.40
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Pig Myostatin (MSTN). This antibody is labeled with HRP.

Myostatin (MSTN) Polyclonal Antibody (Human), HRP

4-PAB653Ra01-HRP
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Myostatin (MSTN). This antibody is labeled with HRP.

Prekallikrein (PK) Polyclonal Antibody (Human), HRP

4-PAB801Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Prekallikrein (PK). This antibody is labeled with HRP.

Versican (VS) Polyclonal Antibody (Human), HRP

4-PAB817Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Versican (VS). This antibody is labeled with HRP.

Versican (VS) Polyclonal Antibody (Mouse), HRP

4-PAB817Mu01-HRP
  • EUR 373.20
  • EUR 3350.40
  • EUR 949.20
  • EUR 469.20
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Versican (VS). This antibody is labeled with HRP.

Vinculin (VCL) Polyclonal Antibody (Human), HRP

4-PAB839Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with HRP.

Paxillin (Pax) Polyclonal Antibody (Human), HRP

4-PAB844Hu01-HRP
  • EUR 366.00
  • EUR 3256.80
  • EUR 926.40
  • EUR 459.60
  • EUR 243.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Paxillin (Pax). This antibody is labeled with HRP.

Nitrotyrosine (NT) Polyclonal Antibody (General), HRP

4-PAB863Ge01-HRP
  • EUR 362.40
  • EUR 3182.40
  • EUR 907.20
  • EUR 452.40
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Nitrotyrosine (NT). This antibody is labeled with HRP.

Aggrecan (AGC) Polyclonal Antibody (Bovine), HRP

4-PAB908Bo01-HRP
  • EUR 410.40
  • EUR 3874.80
  • EUR 1081.20
  • EUR 522.00
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Aggrecan (AGC). This antibody is labeled with HRP.

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